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chondrogenic differentiation medium diff  (ATCC)


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    ATCC chondrogenic differentiation medium diff
    Chondrogenic Differentiation Medium Diff, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chondrogenic differentiation medium diff/product/ATCC
    Average 92 stars, based on 27 article reviews
    chondrogenic differentiation medium diff - by Bioz Stars, 2026-03
    92/100 stars

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    qRT-PCR primers.

    Journal: Biomedicines

    Article Title: Mesenchymal Stem Cells Isolated from Paediatric Paravertebral Adipose Tissue Show Strong Osteogenic Potential

    doi: 10.3390/biomedicines10020378

    Figure Lengend Snippet: qRT-PCR primers.

    Article Snippet: Chondrogenic differentiation of ADMSC was induced in the chondrogenic medium (Chondrocyte Differentiation Tool, ATCC ® PCS-500-051TM, Manassas, VA, USA).

    Techniques: Marker, Control

    Chondrogenic differentiation of human ADMSCs. ( a ) The mRNA expression of ACAN , COL2A1 and COL10A1 in human ADMSCs after 21 days of differentiation. The results are normalized to the control (undifferentiated) PCS-500-011 cells on day 21 ( n = 2). ( b ) Furthermore, after 21 days of incubation in either standard (upper panel) or differentiation medium (lower panel), cells were fixed and stained with Alcian Blue staining solution for glycosaminoglycans.

    Journal: Biomedicines

    Article Title: Mesenchymal Stem Cells Isolated from Paediatric Paravertebral Adipose Tissue Show Strong Osteogenic Potential

    doi: 10.3390/biomedicines10020378

    Figure Lengend Snippet: Chondrogenic differentiation of human ADMSCs. ( a ) The mRNA expression of ACAN , COL2A1 and COL10A1 in human ADMSCs after 21 days of differentiation. The results are normalized to the control (undifferentiated) PCS-500-011 cells on day 21 ( n = 2). ( b ) Furthermore, after 21 days of incubation in either standard (upper panel) or differentiation medium (lower panel), cells were fixed and stained with Alcian Blue staining solution for glycosaminoglycans.

    Article Snippet: Chondrogenic differentiation of ADMSC was induced in the chondrogenic medium (Chondrocyte Differentiation Tool, ATCC ® PCS-500-051TM, Manassas, VA, USA).

    Techniques: Expressing, Control, Incubation, Staining

    Chondrogenic differentiation in alginate encapsulated human LA-ADMSCs. ( a ) Bright-field microscopic pictures of cells encapsulated in alginate. The upper panel shows cells grown in MSCBM and the lower panel in the chondrocyte differentiation medium. ( b ) Immuno-stained differentiated cell aggregates were stained for aggrecan (upper panel) and type II collagen (lower panel) and assessed by fluorescent microscopy.

    Journal: Biomedicines

    Article Title: Mesenchymal Stem Cells Isolated from Paediatric Paravertebral Adipose Tissue Show Strong Osteogenic Potential

    doi: 10.3390/biomedicines10020378

    Figure Lengend Snippet: Chondrogenic differentiation in alginate encapsulated human LA-ADMSCs. ( a ) Bright-field microscopic pictures of cells encapsulated in alginate. The upper panel shows cells grown in MSCBM and the lower panel in the chondrocyte differentiation medium. ( b ) Immuno-stained differentiated cell aggregates were stained for aggrecan (upper panel) and type II collagen (lower panel) and assessed by fluorescent microscopy.

    Article Snippet: Chondrogenic differentiation of ADMSC was induced in the chondrogenic medium (Chondrocyte Differentiation Tool, ATCC ® PCS-500-051TM, Manassas, VA, USA).

    Techniques: Staining, Microscopy

    Cells harvested on day seven from the bioreactor and cultured out in respective differentiation media following specialized protocols. The top row shows osteocyte, the middle row shows adipocyte, and the bottom row shows chondrocyte inductions. After specified time points based on the individual ATCC protocols cells were fixed, stained, and imaged using light microscopy. Scale bars are 100μm. The first column shows stained negative controls of respective cultures.

    Journal: PLoS ONE

    Article Title: Novel low shear 3D bioreactor for high purity mesenchymal stem cell production

    doi: 10.1371/journal.pone.0252575

    Figure Lengend Snippet: Cells harvested on day seven from the bioreactor and cultured out in respective differentiation media following specialized protocols. The top row shows osteocyte, the middle row shows adipocyte, and the bottom row shows chondrocyte inductions. After specified time points based on the individual ATCC protocols cells were fixed, stained, and imaged using light microscopy. Scale bars are 100μm. The first column shows stained negative controls of respective cultures.

    Article Snippet: Cells were resuspended in chondrocyte differentiation toolkit (ATCC PCS-500-051) at 1.25x10 6 cells mL -1 .

    Techniques: Cell Culture, Staining, Light Microscopy